Ryšavá Alena (PhD, MSc.)
Department of Medical Chemistry and Biochemistry
Hněvotínská 3
775 15 Olomouc, Czech Republic
e-mail: rysava.alena@hotmail.com
tel: +420 585 63 2304
Research Topics:
- Phototoxic and photoprotective effects of natural substances, plant extracts (in vitro)
- UVA radiation, oxidative stress, Nrf2 signalling pathway
- Primary human cell cultures (skin fibroblasts, keratinocytes, HUVEC)
- Cell lines (HaCaT), human skin explants
Position:
2022 – Present
Junior researcher, Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Olomouc
Education and postgraduate training:
2015 – 2022
PhD – Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Olomouc
The topic of thesis: Study of phototoxic and photoprotective effects of natural substances
2010 – 2015
Master’s Degree – Department of Cell Biology and Genetics, Faculty of Science, Palacky University, Olomouc
The topic of thesis: Development and characterization of materials for high-density deletion mapping of wheat chromosome 4A
Membership:
2016 – present
Czech Society for Biochemistry and Molecular Biology
Teaching activites:
2016 – present
Biochemistry (Faculty of Medicine and Dentistry, Palacky University, Olomouc)
2015 – 2016
Medical chemistry (Faculty of Medicine and Dentistry, Palacky University, Olomouc)
Grant Projects:
2023 – Present
GACR 23-04654S Chemoenzymatic preparation and biological activity of metabolites of polyphenol compounds from food
2015 – 2017
GACR 15-10897S Modulation of human skin cell responses to UVA radiation by silymarin components
Publications:
· Original papers (5)
· Review (1)
· 5 most important:
1. Ryšavá A., Čížková K., Franková J., Roubalová L., Ulrichová J., Vostálová J., Vrba J., Zálešák B., Rajnochová Svobodová A: Effect of UVA radiation on the Nrf2 signalling pathway in human skin cells. Journal of Photochemistry & Photobiology, B: Biology. 209:111948 (2020). DOI: 10.1016/j.jphotobiol.2020.111948. IF = 6,252.
2. Ryšavá A., Vostálová J., Rajnochová Svobodová A.: Effect of ultraviolet radiation on the Nrf2 signalling pathway in skin cells. International Journal of Radiation Biology. (2021) 97(10) 1383-1403. DOI: 10.1080/09553002.2021.1962566. IF = 2,694.
3. Kosina P., Ryšavá A., Vostálová J., Papoušková B., Biedermann D., Ulrichová J., Svobodová AR.: Stability and ultraviolet A photostability of silymarin polyphenols and its consequences for practical use in dermatology. Journal of Photochemistry and Photobiology A: chemistry. (2022). 429:113897. DOI: 10.1016/j.jphotochem.2022.113897. IF = 5,141.
4. Svobodová AR., Ryšavá A., Čížková K., Roubalová L., Ulrichová J., Vrba J., Zálešák B., Vostálová J.: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts. Photochemical and Photobiological Sciences. (2022). 21:59-75. DOI: 10.1007/s43630-021-00140-9. IF = 4,328.
5. Rajnochová Svobodová A., Gabrielová E., Michaelides L., Kosina P., Ryšavá A., Ulrichová J., Zálešák B., Vostálová J.: UVA-photoprotective potential of silymarin and silybin. Archives of Dermatological Research. (2018) 310(5):413-424. DOI: 10.1007/s00403-018-1828-6. IF = 2,148.
Recent Active and Invited Presentations:
Ryšavá A.: Nrf2 signalling pathway in response to UVA radiation and its modulation by phytochemicals from Silybum marianum in human skin cells. EMBO-FEBS lecture course Molecular mechanisms in signal transduction and cancer. 16. – 24. 8. 2022, Spetses, Greece.
Ryšavá A., Čížková K., Roubalová L., Ulrichová J., Vostálová J., Zálešák B., Rajnochová Svobodová A.: Effects of quercetin and taxifolin on Nrf2 pathway in UVA exposed human skin cells. 45th FEBS congress (Federation of European Biochemical Societies). 3. – 8. 7. 2021, Ljubljana, Slovenia.
Ryšavá A., Biedermann D., Zálešák B., Ulrichová J., Vostálová J., Rajnochová Svobodová A.: Effect of polyphenols from Silybum marianum L. and UVA radiation on Nrf2 signalling pathway in skin cells. 43rd FEBS congress (Federation of European Biochemical Societies). 7. – 12. 7. 2018, Prague, The Czech Republic.
Ryšavá A., Ingremeau M., Biedermann D., Rajnochová Svobodová A., Ulrichová J., Vostálová J., Zálešák B.: Effect of polyphenols from milk thistle (Silybum marianum L.) on Nrf-2 signalling pathway in human skin cells. XVIII. Meeting of biochemists and molecular biologists. 14. – 15. 11. 2017, Brno, Prague, The Czech Republic.
Ryšavá A., Rajnochová Svobodová A., Psotová M., Zálešák B., Ulrichová J., Vostálová J.: The phototoxic potential of taxifolin and quercetin. 19th ISANH International Conference on Oxidative Stress Reduction, Redox Homeostasis & Antioxidants. 26. – 27. 6. 2017, Paris, France.
Recent Awards:
2022
Prize for scientific publication awarded by Dean of Faculty of Medicine, Palacky University Olomouc (2021) for Ryšavá A., Vostálová J., Rajnochová Svobodová A.: Effect of ultraviolet radiation on the Nrf2 signalling pathway in skin cells. Int. J. Radiat. Biol. 97(10) 1383-1403.
2021
Prize for scientific publication awarded by Dean of Faculty of Medicine, Palacky University Olomouc (2020) for Ryšavá A., Čížková K., Franková J., Roubalová L., Ulrichová J., Vostálová J., Vrba J., Zálešák B., Rajnochová Svobodová A: Effect of UVA radiation on the Nrf2 signalling pathway in human skin cells. J. Photochem. Photobiol. B: Biology. 209:111948.